Parallel and patterned optogenetic manipulation of neurons in the brain slice using a DMD-based projector

Seiichiro Sakai, Kenichi Ueno, Toru Ishizuka, Hiromu Yawo

    Research output: Contribution to journalArticlepeer-review

    27 Citations (Scopus)

    Abstract

    Optical manipulation technologies greatly advanced the understanding of the neuronal network and its dysfunctions. To achieve patterned and parallel optical switching, we developed a microscopic illumination system using a commercial DMD-based projector and a software program. The spatiotemporal patterning of the system was evaluated using acute slices of the hippocampus. The neural activity was optically manipulated, positively by the combination of channelrhodopsin-2 (ChR2) and blue light, and negatively by the combination of archaerhodopsin-T (ArchT) and green light. It is suggested that our projector-managing optical system (PMOS) would effectively facilitate the optogenetic analyses of neurons and their circuits.

    Original languageEnglish
    Pages (from-to)59-64
    Number of pages6
    JournalNeuroscience Research
    Volume75
    Issue number1
    DOIs
    Publication statusPublished - 2013 Jan

    Keywords

    • Archaerhodopsin
    • Brain slice
    • Channelrhodopsin
    • Light stimulation
    • Microscope
    • Optics
    • Optogenetics
    • Software

    ASJC Scopus subject areas

    • Neuroscience(all)

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