Abstract
Optical manipulation technologies greatly advanced the understanding of the neuronal network and its dysfunctions. To achieve patterned and parallel optical switching, we developed a microscopic illumination system using a commercial DMD-based projector and a software program. The spatiotemporal patterning of the system was evaluated using acute slices of the hippocampus. The neural activity was optically manipulated, positively by the combination of channelrhodopsin-2 (ChR2) and blue light, and negatively by the combination of archaerhodopsin-T (ArchT) and green light. It is suggested that our projector-managing optical system (PMOS) would effectively facilitate the optogenetic analyses of neurons and their circuits.
Original language | English |
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Pages (from-to) | 59-64 |
Number of pages | 6 |
Journal | Neuroscience Research |
Volume | 75 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2013 Jan |
Keywords
- Archaerhodopsin
- Brain slice
- Channelrhodopsin
- Light stimulation
- Microscope
- Optics
- Optogenetics
- Software
ASJC Scopus subject areas
- Neuroscience(all)