TY - JOUR
T1 - Paradoxically high resistance of natural killer T (NKT) cell-deficient mice to Legionella pneumophila
T2 - Another aspect of NKT cells for modulation of host responses
AU - Hayakawa, Kayoko
AU - Tateda, Kazuhiro
AU - Fuse, Etsu T.
AU - Matsumoto, Tetsuya
AU - Akasaka, Yoshikiyo
AU - Ishii, Toshiharu
AU - Nakayama, Toshinori
AU - Taniguchi, Masaru
AU - Kaku, Mitsuo
AU - Standiford, Theodore J.
AU - Yamaguchi, Keizo
PY - 2008/11
Y1 - 2008/11
N2 - In the present study, we examined the roles of natural killer T (NKT) cells in host defence against Legionella pneumophila in a mouse model. The survival rate of NKT cell-deficient Jα281 knock-out (KO) mice was significantly higher than that of wild-type mice. There was no bacterial overgrowth in the lungs, but Jα281 KO mice showed enhanced pulmonary clearance at a later stage of infection, compared with their wild-type counterparts. The severity of lung injury in L. pneumophila-infected Jα281 KO mice was less, as indicated by lung permeability measurements, such as lung weight and bronchoalveolar lavage fluid albumin concentration. Recruitment of inflammatory cells in the lungs was approximately twofold greater in Jα281 KO mice on day 3. Interestingly, higher values of interleukin (IL)-1β and IL-18, and increased caspase-1 activity were noted in the lungs of Jα281 KO mice from an early time point (6 h). Exogenous α-galactosylceramide, a ligand of NKT cells, induced IL-12 and gamma interferon at 6 h, but suppressed IL-1β at later time points in wild-type, whereas no effects were evident in Jα281 KO mice, as expected. Systemic administration of heat-killed L. pneumophila, but not Escherichia coli LPS, reproduced exaggerated production of IL-1β in the lungs of Jα281 KO mice. These results demonstrate that NKT cells play a role in host defence against L. pneumophila, which is characterized by enhanced lung injury and decreased accumulation of inflammatory cells in the lungs. The regulation of IL-1β, IL-18 and caspase-1 may be associated with the modulating effect of host responses by NKT cells.
AB - In the present study, we examined the roles of natural killer T (NKT) cells in host defence against Legionella pneumophila in a mouse model. The survival rate of NKT cell-deficient Jα281 knock-out (KO) mice was significantly higher than that of wild-type mice. There was no bacterial overgrowth in the lungs, but Jα281 KO mice showed enhanced pulmonary clearance at a later stage of infection, compared with their wild-type counterparts. The severity of lung injury in L. pneumophila-infected Jα281 KO mice was less, as indicated by lung permeability measurements, such as lung weight and bronchoalveolar lavage fluid albumin concentration. Recruitment of inflammatory cells in the lungs was approximately twofold greater in Jα281 KO mice on day 3. Interestingly, higher values of interleukin (IL)-1β and IL-18, and increased caspase-1 activity were noted in the lungs of Jα281 KO mice from an early time point (6 h). Exogenous α-galactosylceramide, a ligand of NKT cells, induced IL-12 and gamma interferon at 6 h, but suppressed IL-1β at later time points in wild-type, whereas no effects were evident in Jα281 KO mice, as expected. Systemic administration of heat-killed L. pneumophila, but not Escherichia coli LPS, reproduced exaggerated production of IL-1β in the lungs of Jα281 KO mice. These results demonstrate that NKT cells play a role in host defence against L. pneumophila, which is characterized by enhanced lung injury and decreased accumulation of inflammatory cells in the lungs. The regulation of IL-1β, IL-18 and caspase-1 may be associated with the modulating effect of host responses by NKT cells.
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U2 - 10.1099/jmm.0.47747-0
DO - 10.1099/jmm.0.47747-0
M3 - Article
C2 - 18927410
AN - SCOPUS:55549145096
VL - 57
SP - 1340
EP - 1348
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
SN - 0022-2615
IS - 11
ER -