TY - JOUR
T1 - Papillary collecting tubule responsiveness to vasoactive hormones in spontaneously hypertensive rats
AU - Sato, Makito
AU - Abe, Keishi
AU - Takeuchi, Kazuhisa
AU - Yasujima, Minoru
AU - Fang, Shounan
AU - Kanazawa, Masayuki
AU - Yoshida, Kazunori
AU - Yoshinaga, Kaoru
PY - 1988/12
Y1 - 1988/12
N2 - Renal papillary collecting tubule (RPCT) hormone responsiveness was compared between cultured RPCT cells from spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Unstimulated cells from 4-week-old SHR produced less prostaglandin E2(PGE2) and cyclic (c)AMP than comparable cells from WKY, while cells from both strains synthesized similar amounts of PGE2after stimulation with arachidonate, A23187 or bradykinin and similar amounts of cAMP after stimulation with vasopressin or PGE2. There was no difference in basai or stimulated levels of cyclic (c)GMP between the strains. In RPCT cells from 16-week-old rats, basal levels of cAMP, cGMP and PGE2were significantly lower than those from 4-week-old rats, but they did not differ between the strains. These results suggest that RPCT cells of SHR and WKY at the post-weaning period may differ in the metabolism of PGE2and cAMP. This difference may be attributed to the possible defect in arachidonate availability in SHR.
AB - Renal papillary collecting tubule (RPCT) hormone responsiveness was compared between cultured RPCT cells from spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Unstimulated cells from 4-week-old SHR produced less prostaglandin E2(PGE2) and cyclic (c)AMP than comparable cells from WKY, while cells from both strains synthesized similar amounts of PGE2after stimulation with arachidonate, A23187 or bradykinin and similar amounts of cAMP after stimulation with vasopressin or PGE2. There was no difference in basai or stimulated levels of cyclic (c)GMP between the strains. In RPCT cells from 16-week-old rats, basal levels of cAMP, cGMP and PGE2were significantly lower than those from 4-week-old rats, but they did not differ between the strains. These results suggest that RPCT cells of SHR and WKY at the post-weaning period may differ in the metabolism of PGE2and cAMP. This difference may be attributed to the possible defect in arachidonate availability in SHR.
KW - Arachidonic acid
KW - Cyclic adenosine monophosphate
KW - Cyclic guanosine monophosphate
KW - Papillary collecting tubule
KW - Prostaglandin E
KW - Spontaneously hypertensive rat
KW - Vasopressin
KW - Wistar-Kyoto rat
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U2 - 10.1097/00004872-198812040-00150
DO - 10.1097/00004872-198812040-00150
M3 - Article
C2 - 2853755
AN - SCOPUS:0024212922
VL - 6
SP - S476-S478
JO - Journal of Hypertension, Supplement
JF - Journal of Hypertension, Supplement
SN - 0952-1178
IS - 4
ER -