Pancreatic beta-cell replication and amelioration of surgical diabetes by Reg protein

Takuo Watanabe; Yutaka Yonemura; Hideto Yonekura; Yoshihisa Suzuki; Hikari Miyashita; Kazuo Sugiyama; Shigeki Moriizumi; Michiaki Unno; Osamu Tanaka; Hisatake Kondo; Adrian J. Bone; Shin Takasawa; Hiroshi Okamoto

doi:10.1073/pnas.91.9.3589

Pancreatic beta-cell replication and amelioration of surgical diabetes by Reg protein

Takuo Watanabe, Yutaka Yonemura, Hideto Yonekura, Yoshihisa Suzuki, Hikari Miyashita, Kazuo Sugiyama, Shigeki Moriizumi, Michiaki Unno, Osamu Tanaka, Hisatake Kondo, Adrian J. Bone, Shin Takasawa, Hiroshi Okamoto

Research output: Contribution to journal › Article › peer-review

236 Citations (Scopus)

Abstract

We previously isolated from a rat regenerating islet cDNA library a gene named Reg, which is expressed in regenerating islets but is not expressed in normal islets. Here we examined the effect of rat Reg protein on pancreatic beta-cell replication using both 90% depancreatized rats and isolated islets. The depancreatized rats that received i.p. administration of recombinant rat Reg protein (1 mg/kg per day) for 2 months showed amelioration of the surgical diabetes, as evidenced by a significant decrease in blood glucose with an increased beta-cell mass in the residual pancreas. In isolated rat islets. Reg protein (18-180 nM: 0.3-3 μg/ml) significantly increased [³H]thymidine incorporation into the nuclei of beta cells. These results indicate that Reg protein is a growth factor for pancreatic beta cells and also suggest that the administration of Reg protein could be used as another therapeutic approach for diabetes mellitus.

Original language	English
Pages (from-to)	3589-3592
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	91
Issue number	9
DOIs	https://doi.org/10.1073/pnas.91.9.3589
Publication status	Published - 1994 Apr 26

Keywords

Reg gene
diabetes mellitus
growth factor
islets of Langerhans

ASJC Scopus subject areas

General

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1073/pnas.91.9.3589

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Watanabe, T., Yonemura, Y., Yonekura, H., Suzuki, Y., Miyashita, H., Sugiyama, K., Moriizumi, S., Unno, M., Tanaka, O., Kondo, H., Bone, A. J., Takasawa, S., & Okamoto, H. (1994). Pancreatic beta-cell replication and amelioration of surgical diabetes by Reg protein. Proceedings of the National Academy of Sciences of the United States of America, 91(9), 3589-3592. https://doi.org/10.1073/pnas.91.9.3589

Watanabe, T, Yonemura, Y, Yonekura, H, Suzuki, Y, Miyashita, H, Sugiyama, K, Moriizumi, S, Unno, M, Tanaka, O, Kondo, H, Bone, AJ, Takasawa, S & Okamoto, H 1994, 'Pancreatic beta-cell replication and amelioration of surgical diabetes by Reg protein', Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 9, pp. 3589-3592. https://doi.org/10.1073/pnas.91.9.3589

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abstract = "We previously isolated from a rat regenerating islet cDNA library a gene named Reg, which is expressed in regenerating islets but is not expressed in normal islets. Here we examined the effect of rat Reg protein on pancreatic beta-cell replication using both 90% depancreatized rats and isolated islets. The depancreatized rats that received i.p. administration of recombinant rat Reg protein (1 mg/kg per day) for 2 months showed amelioration of the surgical diabetes, as evidenced by a significant decrease in blood glucose with an increased beta-cell mass in the residual pancreas. In isolated rat islets. Reg protein (18-180 nM: 0.3-3 μg/ml) significantly increased [3H]thymidine incorporation into the nuclei of beta cells. These results indicate that Reg protein is a growth factor for pancreatic beta cells and also suggest that the administration of Reg protein could be used as another therapeutic approach for diabetes mellitus.",

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AB - We previously isolated from a rat regenerating islet cDNA library a gene named Reg, which is expressed in regenerating islets but is not expressed in normal islets. Here we examined the effect of rat Reg protein on pancreatic beta-cell replication using both 90% depancreatized rats and isolated islets. The depancreatized rats that received i.p. administration of recombinant rat Reg protein (1 mg/kg per day) for 2 months showed amelioration of the surgical diabetes, as evidenced by a significant decrease in blood glucose with an increased beta-cell mass in the residual pancreas. In isolated rat islets. Reg protein (18-180 nM: 0.3-3 μg/ml) significantly increased [3H]thymidine incorporation into the nuclei of beta cells. These results indicate that Reg protein is a growth factor for pancreatic beta cells and also suggest that the administration of Reg protein could be used as another therapeutic approach for diabetes mellitus.

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Pancreatic beta-cell replication and amelioration of surgical diabetes by Reg protein

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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