Pancreatic beta-cell replication and amelioration of surgical diabetes by Reg protein

Takuo Watanabe, Yutaka Yonemura, Hideto Yonekura, Yoshihisa Suzuki, Hikari Miyashita, Kazuo Sugiyama, Shigeki Moriizumi, Michiaki Unno, Osamu Tanaka, Hisatake Kondo, Adrian J. Bone, Shin Takasawa, Hiroshi Okamoto

Research output: Contribution to journalArticlepeer-review

236 Citations (Scopus)


We previously isolated from a rat regenerating islet cDNA library a gene named Reg, which is expressed in regenerating islets but is not expressed in normal islets. Here we examined the effect of rat Reg protein on pancreatic beta-cell replication using both 90% depancreatized rats and isolated islets. The depancreatized rats that received i.p. administration of recombinant rat Reg protein (1 mg/kg per day) for 2 months showed amelioration of the surgical diabetes, as evidenced by a significant decrease in blood glucose with an increased beta-cell mass in the residual pancreas. In isolated rat islets. Reg protein (18-180 nM: 0.3-3 μg/ml) significantly increased [3H]thymidine incorporation into the nuclei of beta cells. These results indicate that Reg protein is a growth factor for pancreatic beta cells and also suggest that the administration of Reg protein could be used as another therapeutic approach for diabetes mellitus.

Original languageEnglish
Pages (from-to)3589-3592
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number9
Publication statusPublished - 1994 Apr 26


  • Reg gene
  • diabetes mellitus
  • growth factor
  • islets of Langerhans

ASJC Scopus subject areas

  • General


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