TY - JOUR
T1 - Pan1b (17βHSD11)-enzymatic activity and distribution in the lung
AU - Brereton, Phillip
AU - Suzuki, Takashi
AU - Sasano, Hironobu
AU - Li, Kevin
AU - Duarte, Carla
AU - Obeyesekere, Varuni
AU - Haeseleer, Francoise
AU - Palczewski, Krzysztof
AU - Smith, Ian
AU - Komesaroff, Paul
AU - Krozowski, Zygmunt
N1 - Funding Information:
We thank Dr R.N. Fariss for help with immunolocalization in the retina, and Dr G. Yang for help with the enzymatic assays. This work was supported by a Block Grant to the Baker Institute from the NHIVIRC of Australia, and by a grant from the National Institute of Health EY08061 to K. Palczewski.
PY - 2001/1/22
Y1 - 2001/1/22
N2 - We describe a new member of the 17β-hydroxysteroid dehydrogenase group of enzymes. Human Pan1b displays greatest activity with 5α-androstan-3α,17β-diol (3α-Diol) as substrate, suggesting that it may be important in androgen metabolism. Enzymic activity was non-saturable with 3α-Diol but saturable with retinoids, although retinoids were not metabolized. Immunohistochemical studies on 10% formalin fixed and paraffin embedded sections of human tissues showed that Pan1b was present in acini and ciliated epithelia of the lung. In the fetus immuno reactivity was present in ciliated epithelia throughout gestation and staining appeared to be stronger in the second half of pregnancy. Pan1b was also expressed in the nonpigmented epithelium of the ciliary body, and in adrenocortical tumor cells. Although 3α-Diol is generally considered a degradation product of androgen metabolism it could have its own biological function. Pan1b may be an important modulator of the endocrine, or intracrine activity of this steroid.
AB - We describe a new member of the 17β-hydroxysteroid dehydrogenase group of enzymes. Human Pan1b displays greatest activity with 5α-androstan-3α,17β-diol (3α-Diol) as substrate, suggesting that it may be important in androgen metabolism. Enzymic activity was non-saturable with 3α-Diol but saturable with retinoids, although retinoids were not metabolized. Immunohistochemical studies on 10% formalin fixed and paraffin embedded sections of human tissues showed that Pan1b was present in acini and ciliated epithelia of the lung. In the fetus immuno reactivity was present in ciliated epithelia throughout gestation and staining appeared to be stronger in the second half of pregnancy. Pan1b was also expressed in the nonpigmented epithelium of the ciliary body, and in adrenocortical tumor cells. Although 3α-Diol is generally considered a degradation product of androgen metabolism it could have its own biological function. Pan1b may be an important modulator of the endocrine, or intracrine activity of this steroid.
KW - 17β-Hydroxysteroid dehydrogenase
KW - 3α-Diol
KW - Adrenal gland
KW - Androgens
KW - Estrogens
KW - Eye
KW - Human lung
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U2 - 10.1016/S0303-7207(00)00417-2
DO - 10.1016/S0303-7207(00)00417-2
M3 - Article
C2 - 11165019
AN - SCOPUS:0035931119
VL - 171
SP - 111
EP - 117
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1-2
ER -