Paenibacillus konkukensis sp. Nov., isolated from animal feed

A Gram-stain-positive, oxidase- and catalase-positive, aerobic, rod-shaped bacterium, designated strain SK-3146^T, was isolated from animal feed. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, revealed that the strain formed a distinct lineage within the genus Paenibacillus that was closely related to Paenibacillus yunnanensis JCM 30953^T (98.6 %), Paenibacillus vulneris CCUG 53270^T (98.0 %) and Paenibacillus chinjuensis DSM 15045^T (96.9 %). Cells were nonmotile, endospore-forming and formed milky colonies on NA and R2A agar media. Growth of strain SK-3146^T occurred at temperatures of 18–45 °C, at pH 6.0–9.5 and between 0.5–3.0%NaCl (w/v). The major menaquinone was MK-7, with lesser amounts of MK-6 present. The cell wall peptidoglycan of strain SK-3146T contained meso-diaminopimelic acid. The major fatty acids were anteiso-C_{15 : 0} and iso-C_{16 : 0}. The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 53.8 mol% and the DNA–DNA hybridization relatedness values between strain SK-3146^T and P. yunnanensis JCM 30953^T and P. vulneris CCUG 53270^T were 26.13±0.8%and 38.7±0.6 %, respectively. The phenotypic, phylogenetic and chemotaxonomic results indicate that strain SK-3146^T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus konkukensis sp. nov. is proposed. The type strain is SK-3146^T (=KACC 18876^T=LMG 29568^T).