Paenibacillus konkukensis sp. Nov., isolated from animal feed

Wan Taek Im, Kwon Jung Yi, Sang Suk Lee, Hyung In Moon, Che Ok Jeon, Dong Woon Kim, Soo Ki Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A Gram-stain-positive, oxidase- and catalase-positive, aerobic, rod-shaped bacterium, designated strain SK-3146T, was isolated from animal feed. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, revealed that the strain formed a distinct lineage within the genus Paenibacillus that was closely related to Paenibacillus yunnanensis JCM 30953T (98.6 %), Paenibacillus vulneris CCUG 53270T (98.0 %) and Paenibacillus chinjuensis DSM 15045T (96.9 %). Cells were nonmotile, endospore-forming and formed milky colonies on NA and R2A agar media. Growth of strain SK-3146T occurred at temperatures of 18–45 °C, at pH 6.0–9.5 and between 0.5–3.0%NaCl (w/v). The major menaquinone was MK-7, with lesser amounts of MK-6 present. The cell wall peptidoglycan of strain SK-3146T contained meso-diaminopimelic acid. The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 53.8 mol% and the DNA–DNA hybridization relatedness values between strain SK-3146T and P. yunnanensis JCM 30953T and P. vulneris CCUG 53270T were 26.13±0.8%and 38.7±0.6 %, respectively. The phenotypic, phylogenetic and chemotaxonomic results indicate that strain SK-3146T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus konkukensis sp. nov. is proposed. The type strain is SK-3146T (=KACC 18876T=LMG 29568T).

Original languageEnglish
Pages (from-to)2343-2348
Number of pages6
JournalInternational journal of systematic and evolutionary microbiology
Volume67
Issue number7
DOIs
Publication statusPublished - 2017 Jul
Externally publishedYes

Keywords

  • Firmicutes
  • Paenibacillus konkukensis
  • Polyphasic taxonomy
  • Taxonomy

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Fingerprint Dive into the research topics of 'Paenibacillus konkukensis sp. Nov., isolated from animal feed'. Together they form a unique fingerprint.

  • Cite this