Abstract
Tetrahymena p85 is localized to the presumptive division plane before the formation of contractile ring microfilaments. p85 binds to calmodulin in a Ca2+-dependent manner and both proteins colocalize to the division furrow. Inhibition of the binding of p85 and Ca2+/calmodulin prevents both the localization of p85 and calmodulin to the division plane and the formation of the contractile ring, suggesting that the interaction of p85 and Ca2+/calmodulin is important in the formation of the contractile ring. We investigated the mechanisms of the formation of contractile ring, and the relationship among p85, CaM, and actin using co-sedimentation assay: p85 binds to G-actin in a Ca2+/calmodulin-dependent manner, but does not bind to F-actin. Therefore, we propose that a Ca2+/calmodulin signal and its target protein p85 are cooperatively involved in the recruitment of G-actin to the division plane and the formation of the contractile ring.
Original language | English |
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Pages (from-to) | 1098-1103 |
Number of pages | 6 |
Journal | Biochemical and biophysical research communications |
Volume | 292 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2002 |
Externally published | Yes |
Keywords
- Actin
- Actin-binding protein
- Ca
- Calmodulin
- Contractile ring
- Cytokinesis
- Division plane
- Microfilament
- Tetrahymena
- p85
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology