P63RhoGEF-mediated formation of a single polarized lamellipodium is required for chemotactic migration in breast carcinoma cells

Aya Hayashi, Ryuichi Hiatari, Takuji Tsuji, Kazumasa Ohashi, Kensaku Mizuno

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Short hairpin RNAs targeting 66 Rho-GEFs were screened for inhibition of chemotaxis. Six Rho-GEFs (p63RhoGEF, Trio, Duet, Net1, Frabin/Fgd4, and AAH33666) were found to be required for the serum-induced chemotactic migration of MDA-MB-231 human breast carcinoma cells. Knockdown of p63RhoGEF suppressed serum-induced RhoA activation and chemotaxis and caused the aberrant formation of multiple lamellipodial protrusions after serum stimulation while control cells formed a single polarized lamellipodium. These results indicate that p63RhoGEF plays a crucial role in serum-induced chemotaxis by limiting lamellipodial protrusion to one direction via RhoA activation.

    Original languageEnglish
    Pages (from-to)698-705
    Number of pages8
    JournalFEBS Letters
    Volume587
    Issue number6
    DOIs
    Publication statusPublished - 2013 Mar 18

    Keywords

    • Cell migration
    • Lamellipodium
    • MDA-MB-231 cell
    • Rho-GEF
    • RhoA
    • p63RhoGEF

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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