P-Selectin and intercellular adhesion Molecule-1 expression after focal brain ischemia and reperfusion

Yasushi Okada, Brian R. Copeland, Etsuro Mori, Ming Ming Tung, Winston S. Thomas, Gregory J. del Zoppo

Research output: Contribution to journalArticlepeer-review

379 Citations (Scopus)


Background and Purpose Polymorphonuclear leukocytes have been implicated in the development of the “no-reflow” phenomenon after focal cerebral ischemia and reperfusion. To further understand the role of granulocytes in microvascular occlusions, the responses of the granulocyte-endothelial cell adhesion molecules P-selectin and intercellular adhesion molecule-1 during middle cerebral artery ischemia and reperfusion were examined in a primate model. Methods Twelve adolescent male baboons were used for 2-hour middle cerebral artery occlusion (n=3) or for 3-hour occlusion with 1-hour (n=3), 4-hour (n=3), and 24-hour (n=3) reperfusion, and three separate unoperated primates served as controls. A quantitative immunohistochemical study of the microvascular distribution of P-selectin and intercellular adhesion molecule-1 was performed using 10-µm frozen sections from basal ganglia analyzed with computerized light microscopy video imaging. Results Significant (P<.05) persistent upregulation of P-selectin (beginning during ischemia) and transient upregulation of intercellular adhesion molecule-1 (at 1 and 4 hours of reperfusion) were observed on endothelium of selected postcapillary microvessels of the ischemic lenticulostriate artery territory. Platelet accumulation also occurred in this territory and was responsible for a significant proportion of the nonendothelial P-selectin signal at 24 hours after reperfusion. Conclusions Focal cerebral ischemia/reperfusion stimulates endothelial P-selectin and intercellular adhesion molecule-1 expression in brain microvessels in the ischemic zone, which may contribute to enhanced leukocyte adherence and persistent activation.

Original languageEnglish
Pages (from-to)202-210
Number of pages9
Issue number1
Publication statusPublished - 1994 Jan
Externally publishedYes


  • Cell adhesion molecules
  • Endothelium
  • Leukocytes
  • Microcirculation
  • Reperfusion

ASJC Scopus subject areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialised Nursing


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