Oxygen equilibrium and electron paramagnetic resonance studies on copper(II)-iron(II) hybrid hemoglobins at room temperature

Naoya Shibayama, Masao Ikeda-Saito, Hiroshi Hori, Kazuya Itaroku, Hideki Morimoto, Satoshi Saigo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Copper(II_-iron(II) hybrid hemoglobins, in which hemes in either the α or β subunits are substituted with copper (II) protoporphyrin IX, have been prepared. The affinities of the ferrous-subunits in both hybrids for the first binding oxygen are as low as the affinity of deoxyhemoglobin under various solution conditions, indicating the equality of behavior in copper(II) protoporphyrin IX and deoxyheme. Electron paramagnetic resonance (EPR) examinations on these hybrids at room temperature show that the interaction between copper(II) and the proximal histidine (F8) is specifically weakened in the α subunits within a low affinity conformation of hemoglobin. These results suggest that copper(II) protoporphyrin IX is a useful EPR probe at room temperature for investigating the deoxyheme environment in hemoglobin.

Original languageEnglish
Pages (from-to)126-130
Number of pages5
JournalFEBS Letters
Volume372
Issue number1
DOIs
Publication statusPublished - 1995 Sep 18

Keywords

  • EPR
  • Hemoglobin
  • Intermediate species
  • Metal-substitution
  • Oxygenation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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