Oxygen consumption of cell suspension in a poly(dimethylsiloxane) (PDMS) microchannel estimated by scanning electrochemical microscopy

Takeshi Saito, Ching Chou Wu, Hitoshi Shiku, Tomoyuki Yasukawa, Masaki Yokoo, Takashi Ito-Sasaki, Hiroyuki Abe, Hiroyoshi Hoshi, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

A quantitative analysis of the oxygen concentration profile near a poly(dimethylsiloxane) (PDMS) microfluidic device was performed using scanning electrochemical microscopy (SECM). A microchannel filled with sodium sulfite (Na2SO3) aqueous solution was imaged by SECM, showing that the oxygen diffusion layer of the PDMS microchannel was observed to be hemicylindrical. Based on a theoretical analysis of the hemicylindrical diffusion layer of the microchannel, the total oxygen mass transfer rates of oxygen to the PDMS microchannel filled with the Na2SO3 solution was calculated to be (4.01 ± 0.30) × 10-12 mol s-1. This is the maximum value of the oxygen transfer rate for this PDMS microchannel device. The oxygen consumption rate increased almost linearly with the logarithm of the concentration of E. coli cells (106∼ 108 cells). The respiratory activity for a single E. coli cell was estimated to be ∼4.31 × 10-20 mol s-1 cell -1.

Original languageEnglish
Pages (from-to)1006-1011
Number of pages6
JournalAnalyst
Volume131
Issue number9
DOIs
Publication statusPublished - 2006 Jan 1

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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