TY - JOUR
T1 - Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome
AU - Ngo, Kien Xuan
AU - Umakoshi, Hiroshi
AU - Ishii, Haruyuki
AU - Bui, Huong Thi
AU - Shimanouchi, Toshinori
AU - Kuboi, Ryoichi
N1 - Funding Information:
The fundamental concept of this study was supported by the Research Group of “Membrane Stress Biotechnology.” It was supported in part by a Grant-in-Aid for Scientific Research (No. 17656268, 19656203, 19656220, 20360350, and 21246121) from the Ministry of Education, Science, Sports, and Culture of Japan, a grant from the 21st Century COE program “Creation of Integrated EcoChemistry” and the Global COE program “Bio-Environmental Chemistry” of the Japan Society for the Promotion of Science (JSPS). The authors are grateful to the Research Center for Solar Energy Chemistry of Osaka University and the Gas hydrate Analyzing System of Osaka University. K. X. Ngo and H. T. Bui acknowledge financial support from the Ministry of Education and Training, Vietnam (MOET) and the Japanese Society of Promotion of Science (JSPS).
PY - 2009/12
Y1 - 2009/12
N2 - The effective production and secretion of chitosanase from S. griseus cells, in the presence of hydrogen peroxide, were studied by the treatment of the cells with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes under heat condition. The variation of the conformation and activity of the chitosanase caused by the interaction of liposomes with target chitosanase under stress condition was systematically investigated by using circular dichroism (CD) spectra and dielectric dispersion analysis (DDA). The effect of the oxidative stress (hydrogen peroxide) on the lipid and protein peroxide of cell membrane of S. griseus pretreated with and without liposomes under heat stress at 41 °C was further carried out. The possible utilization of membrane-membrane interaction between liposomes and cell membrane induced by the heat treatment was further investigated to enhance the production of chitosanase from S. griseus under oxidative stress condition.
AB - The effective production and secretion of chitosanase from S. griseus cells, in the presence of hydrogen peroxide, were studied by the treatment of the cells with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes under heat condition. The variation of the conformation and activity of the chitosanase caused by the interaction of liposomes with target chitosanase under stress condition was systematically investigated by using circular dichroism (CD) spectra and dielectric dispersion analysis (DDA). The effect of the oxidative stress (hydrogen peroxide) on the lipid and protein peroxide of cell membrane of S. griseus pretreated with and without liposomes under heat stress at 41 °C was further carried out. The possible utilization of membrane-membrane interaction between liposomes and cell membrane induced by the heat treatment was further investigated to enhance the production of chitosanase from S. griseus under oxidative stress condition.
KW - Chitosanase
KW - Liposome
KW - Membrane stress biotechnology
KW - Oxidative stress
KW - Streptomyces griseus
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UR - http://www.scopus.com/inward/citedby.url?scp=70449368214&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2009.06.010
DO - 10.1016/j.jbiosc.2009.06.010
M3 - Article
C2 - 19914578
AN - SCOPUS:70449368214
VL - 108
SP - 471
EP - 476
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 6
ER -