Oxidative stress induced lipocalin 2 gene expression: Addressing its expression under the harmful conditions

Mehryar Habibi Roudkenar, Yoshikazu Kuwahara, Taisuke Baba, Amaneh Mohammadi Roushandeh, Shigeko Ebishima, Shinya Abe, Yasuhito Ohkubo, Manabu Fukumoto

Research output: Contribution to journalArticlepeer-review

131 Citations (Scopus)


Lipocalin 2 (Lcn2, NGAL) is a member of the lipocalin superfamily with diverse functions such as the transport of fatty acids and the induction of apoptosis. Previous reports indicated that expression of Lcn2 is induced under harmful conditions. However, the mechanisms of the induction of Lcn2 expression remain to be elucidated. In this report, we intended to identify the factor or factors that induce Lcn2 expression. Up-regulation of Lcn2 expression after X-ray exposure was detected in the heart, the kidney and especially in the liver. Primary culture of liver component cells revealed that this up-regulation in the liver was induced in hepatocytes. Up-regulation of Lcn2 expression was also detected in HepG2 cells after the administration of X-rays or H 2O2. Interestingly, up-regulation of Lcn2 expression after H2O2 treatment was canceled by the addition of the anti-oxidants, dimethylsulfoxide or cysteamine. These results strongly suggest that Lcn2 expression is induced by reactive oxygen species. Therefore, Lcn2 could be a useful biomarker to identify oxidative stress both in vitro and in vivo.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalJournal of radiation research
Issue number1
Publication statusPublished - 2007
Externally publishedYes


  • HO
  • Lipocalin 2
  • Liver
  • Oxidative stress
  • Radiation

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis


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