OX40 ligand regulates splenic CD8- dendritic cell-induced Th2 responses in vivo

Fumitaka Kamachi, Norihiro Harada, Yoshihiko Usui, Tamami Sakanishi, Naoto Ishii, Ko Okumura, Sachiko Miyake, Hisaya Akiba

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In mice, splenic conventional dendritic cells (cDCs) can be separated, based on their expression of CD8α into CD8- and CD8+ cDCs. Although previous experiments demonstrated that injection of antigen (Ag)-pulsed CD8- cDCs into mice induced CD4 T cell differentiation toward Th2 cells, the mechanism involved is unclear. In the current study, we investigated whether OX40 ligand (OX40L) on CD8- cDCs contributes to the induction of Th2 responses by Ag-pulsed CD8- cDCs in vivo, because OX40-OX40L interactions may play a preferential role in Th2 cell development. When unseparated Ag-pulsed OX40L-deficient cDCs were injected into syngeneic BALB/c mice, Th2 cytokine (IL-4, IL-5, and IL-10) production in lymph node cells was significantly reduced. Splenic cDCs were separated to CD8 - and CD8+ cDCs. OX40L expression was not observed on freshly isolated CD8- cDCs, but was induced by anti-CD40 mAb stimulation for 24 h. Administration of neutralizing anti-OX40L mAb significantly inhibited IL-4, IL-5, and IL-10 production induced by Ag-pulsed CD8- cDC injection. Moreover, administration of anti-OX40L mAb with Ag-pulsed CD8- cDCs during a secondary response also significantly inhibited Th2 cytokine production. Thus, OX40L on CD8- cDCs physiologically contributes to the development of Th2 cells and secondary Th2 responses induced by Ag-pulsed CD8- cDCs in vivo.

Original languageEnglish
Pages (from-to)235-240
Number of pages6
JournalBiochemical and biophysical research communications
Volume444
Issue number2
DOIs
Publication statusPublished - 2014 Feb 7

Keywords

  • Costimulation
  • Dendritic cell
  • OX40 ligand
  • Th2 response

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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