Overexpression of the novel F-box protein Ymr258c confers resistance to methylmercury in Saccharomyces cerevisiae

Gi Wook Hwang, Naomi Wada, Shusuke Kuge, Akira Naganuma

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

We searched a yeast genomic library for genes that conferred methylmercury resistance to yeast. Ymr258c was identified as a factor that conferred strong methylmercury resistance when overex-pressed in budding yeast. Ymr258c is regarded as one of the F-box proteins, i.e., component factors of the Skp1/Cullin/F-box protein (SCF) complex that functions as a ubiquitin ligase in the ubiquitin-proteasome system. In this study, Ymr258c was demonstrated to function as an F-box protein to reduce methylmercu-ry toxicity. The overexpression of Ymr258c might promote the ubiquitination of proteins that are involved in the enhancement of methylmercury toxicity, and thereby promote their degradation by proteasomes to reduce methylmercury toxicity.

Original languageEnglish
Pages (from-to)413-416
Number of pages4
JournalJournal of Toxicological Sciences
Volume34
Issue number4
DOIs
Publication statusPublished - 2009 Aug

Keywords

  • F-box protein
  • Methylmercury
  • Resistance
  • Yeast
  • Ymr258c

ASJC Scopus subject areas

  • Toxicology

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