Overexpression of the novel F-box protein Ymr258c confers resistance to methylmercury in Saccharomyces cerevisiae

We searched a yeast genomic library for genes that conferred methylmercury resistance to yeast. Ymr258c was identified as a factor that conferred strong methylmercury resistance when overex-pressed in budding yeast. Ymr258c is regarded as one of the F-box proteins, i.e., component factors of the Skp1/Cullin/F-box protein (SCF) complex that functions as a ubiquitin ligase in the ubiquitin-proteasome system. In this study, Ymr258c was demonstrated to function as an F-box protein to reduce methylmercu-ry toxicity. The overexpression of Ymr258c might promote the ubiquitination of proteins that are involved in the enhancement of methylmercury toxicity, and thereby promote their degradation by proteasomes to reduce methylmercury toxicity.