Overexpression of the barley nicotianamine synthase gene HvNAS1 increases iron and zinc concentrations in rice grains

Hiroshi Masuda, Kanako Usuda, Takanori Kobayashi, Yasuhiro Ishimaru, Yusuke Kakei, Michiko Takahashi, Kyoko Higuchi, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

In humans, iron (Fe) and zinc (Zn) deficiencies result in major worldwide health problems. Transgenic technologies to produce Fe- and Zn-biofortified rice varieties offer a promising potential solution. Nicotianamine, the precursor of phytosiderophores, chelates Fe 2+ and Zn 2+ and plays an important role in transporting these metals to both vegetative and reproductive organs within the plant. Our objective was to increase Fe and Zn contents in rice grains by overexpressing the barley nicotianamine synthase gene HvNAS1. HvNAS1-overexpressing transgenic rice showed increased HvNAS1 expression and subsequent increases in endogenous nicotianamine and phytosiderophore content in shoots, roots, and seeds. Fe and Zn concentrations in polished T 1 seeds from transgenic plants increased more than three and twofold, respectively; Fe and Zn concentrations also increased in both polished and brown T 2 seeds. These results suggest that the overproduction of nicotianamine enhances the translocation of Fe and Zn into rice grains.

Original languageEnglish
Pages (from-to)155-166
Number of pages12
JournalRice
Volume2
Issue number4
DOIs
Publication statusPublished - 2009 Dec 1
Externally publishedYes

Keywords

  • Biofortification
  • HvNAS1
  • Iron
  • Nicotianamine
  • Transgenic rice
  • Zinc

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science
  • Plant Science

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  • Cite this

    Masuda, H., Usuda, K., Kobayashi, T., Ishimaru, Y., Kakei, Y., Takahashi, M., Higuchi, K., Nakanishi, H., Mori, S., & Nishizawa, N. K. (2009). Overexpression of the barley nicotianamine synthase gene HvNAS1 increases iron and zinc concentrations in rice grains. Rice, 2(4), 155-166. https://doi.org/10.1007/s12284-009-9031-1