Overexpression of the AML1 proto-oncoprotein in NIH3T3 cells leads to neoplastic transformation depending on the DNA-binding and transactivational potencies

Mineo Kurokawa, Tomoyuki Tanaka, Kozo Tanaka, Seishi Ogawa, Kinuko Mitani, Yoshio Yazaki, Hisamaru Hirai

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)

Abstract

The AML1 gene encodes DNA-binding proteins that contain the Punt homology domain and is found at the breakpoints of t(8;21) and t(3;21) translocations associated with myelogenous leukemias. From the AML1 gene, two representative forms of proteins, AML1a and AML1b, are generated by alternative splicing. Both forms have the Punt homology domain that possesses the DNA-binding ability but, unlike AML1b, AML1a lacks a putative transcriptional activation domain downstream of the Punt homology domain. By using retroviral infection, we demonstrated that AML1b causes neoplastic transformation of NIH3T3 cells. AML1b-expressing cells form macroscopic colonies in soft agar and induce tumors in nude mice, indicating that AML1 can be a transforming gene when overexpressed in fibroblasts. Both the Punt homology domain and the transactivational domain were required to transform NIH3T3 cells. By analysis of deletion mutants, it was shown that an element determining the transactivational potency exists between amino acids 288 and 396 within the region downstream of the runt homology domain. Furthermore, we demonstrated that this region was also required for fibroblast transformation, indicating that the transforming activity of AML1 is correlated with the transactivational potencies. These results suggest a role of AML1 in the regulation of cellular proliferation, as well as myeloid cell differentiation.

Original languageEnglish
Pages (from-to)883-892
Number of pages10
JournalOncogene
Volume12
Issue number4
Publication statusPublished - 1996 Mar 13
Externally publishedYes

Keywords

  • AML1
  • DNA-binding
  • Fibroblasts
  • Transactivational potencies
  • Transformation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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