Overexpression of manganese-superoxide dismutase prevents methylmercury toxicity in hela cells

Akira Naganuma, Kyoko Miura, Toshiko Tanaka-Kagawa, Jun Kitahara, Yoshiyuki Seko, Haruka Toyoda, Nobumasa Imura

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

HeLa cells were stably transformed with plasmid constructs that allowed constitutive expression of antioxidant enzymes such as catalase, glutathione peroxidase (GSH-Px). Cu,Zn-superoxide dismutase (Cu,Zn-SOD) or Mn-superoxide dismutase (Mn-SOD) to examine the involvement of reactive oxygen generation in methylmercury toxicity. Overexpression of catalase, GSH-Px or Cu,Zn-SOD did not affect the sensitivity of HeLa cells against methylmercury. However, the sensitivity of HeLa cells against methylmercury was decreased by overexpression of Mn-SOD, an enzyme localized in matrix of mitochondria and which decomposes superoxide anions. These results suggest that formation of superoxide anions in the mitochondria might be involved in the mechanism of the cytotoxicity of methylmercury.

Original languageEnglish
JournalLife Sciences
Volume62
Issue number12
DOIs
Publication statusPublished - 1998 Feb 13

Keywords

  • Catalase
  • Cu,Zn-SOD
  • Gene transfection
  • Glutathione peroxidase
  • HeLa cells
  • Methylmercury
  • Mn-SOD

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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