Ovarian polarity and cell shape determination by Btk29A in Drosophila

Noriko Hamada-Kawaguchi, Daisuke Yamamoto

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Drosophila Btk29A is a Tec family nonreceptor tyrosine kinase, the ortholog of which causes X-linked agammaglobulinemia in humans when mutant. In Btk29AficP mutant ovaries, multiple defects are observed: extrapolar cells form ectopically; osk mRNA fails to accumulate posteriorly in mature oocytes; the shape and alignment of follicle cells are grossly distorted. All these phenotypes are rescued by selectively overexpressing the type 2 isoform of wild-type Btk29A in follicle cells. Expression of certain proteins enriched in adherens junctions is markedly affected in Btk29AficP mutants; the anterior–posterior gradient normally observed in the expression of DE-Cadherin and Armadillo are lost and Canoe is sequestered from adherens junctions. Intriguingly, tyrosine phosphorylation of Canoe is reduced in Btk29AficP mutants. It is proposed that Btk29A is required for the establishment of egg chamber polarity presumably through the regulation of subcellular localization of its downstream proteins, including Cno.

    Original languageEnglish
    Article numbere23042
    JournalGenesis
    Volume55
    Issue number8
    DOIs
    Publication statusPublished - 2017 Aug

    Keywords

    • Cadherin
    • Canoe
    • oogenesis
    • oskar
    • β-Catenin

    ASJC Scopus subject areas

    • Genetics
    • Endocrinology
    • Cell Biology

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