OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints

Takahiro Aoyama, Takanori Kobayashi, Michiko Takahashi, Seiji Nagasaka, Kanako Usuda, Yusuke Kakei, Yasuhiro Ishimaru, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa

Research output: Contribution to journalArticlepeer-review

111 Citations (Scopus)

Abstract

Iron uptake and translocation in plants are important processes for both plant and human nutrition, whereas relatively little is known about the molecular mechanisms of iron transport within the plant body. Several reports have shown that yellow stripe 1 (YS1) and YS1-like (YSL) transporters mediate metal-phytosiderophore uptake and/or metal-nicotianamine translocation. Among the 18 YSL genes in rice (OsYSLs), OsYSL18 is predicted to encode a polypeptide of 679 amino acids containing 13 putative transmembrane domains. An OsYSL18-green fluorescent protein (GFP) fusion was localized to the plasma membrane when transiently expressed in onion epidermal cells. Electrophysiological measurements using Xenopus laevis oocytes showed that OsYSL18 transports iron(III)-deoxymugineic acid, but not iron(II)-nicotianamine, zinc(II)-deoxymugineic acid, or zinc(II)-nicotianamine. Reverse transcriptase PCR analysis revealed more OsYSL18 transcripts in flowers than in shoots or roots. OsYSL18 promoter-β-glucuronidase (GUS) analysis revealed that OsYSL18 was expressed in reproductive organs including the pollen tube. In vegetative organs, OsYSL18 was specifically expressed in lamina joints, the inner cortex of crown roots, and phloem parenchyma and companion cells at the basal part of every leaf sheath. These results suggest that OsYSL18 is an iron-phytosiderophore transporter involved in the translocation of iron in reproductive organs and phloem in joints.

Original languageEnglish
Pages (from-to)681-692
Number of pages12
JournalPlant Molecular Biology
Volume70
Issue number6
DOIs
Publication statusPublished - 2009 Aug

Keywords

  • Iron
  • Phytosiderophore
  • Rice
  • Translocation
  • Transporter
  • YS1-like gene

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Fingerprint Dive into the research topics of 'OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints'. Together they form a unique fingerprint.

Cite this