Ostrich intestinal glycohydrolases: Distribution, purification and partial characterisation

Vaughan Oosthuizen, Durand P. Weldrick, Ryno J. Naudé, Willem Oelofsen, Koji Muramoto, Hisao Kamiya

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    9 Citations (Scopus)

    Abstract

    Intestinal glycohydrolases are enzymes involved in assimilating carbohydrate for nutrition. The avian forms of these enzymes, in particular the maltase-glucoamylase complex (MG), are not well characterised. This study encompassed characterisation of these enzymes from ostrich intestines, and the first kinetic analysis of an avian MG, Proteolytically solubilised MG from ileal brush border membrane vesicles was purified by Sephadex G-200 gel filtration and Tris-affinity chromatography, while jejunal sucrase-isomaltase (SI) and MG were purified by Toyopearl-Q650 and phenyl-Sepharose chromatography. Amino acid sequences and compositions of enzyme subunits, resulting from SDS-PAGE, were determined. Kinetics of hydrolysis of linear oligosaccharides was studied. Ostrich MG and SI showed the highest activity in the jejunum, followed by the ileum and duodenum. No lactase or trehalase activity could be detected. The jejunal MG and SI, resulting from brush-border membrane vesicles, could not be separated during purification. However, a minor form of ileal MG was purified using Sephadex G-200 chromatography. Ileal MG contained three subunits of M(r) 145000, 125000 and 115000. Although the N-terminal amino acid sequences bear no homology to SI, the M(r) 115000 subunit shows homology to porcine MG in both sequence and amino acid composition. The pH optimum of maltose-, starch- and isomaltose-hydrolysing activity was 6.5 and that of sucrosehydrolysing activity 5.5. The glycohydrolases were most active at 58°C, but were quickly denatured above 60°C. Sucrose- and starch-hydrolysing activities were more thermostable than maltose- and isomaltose-hydrolysing activities. Kinetic parameters (K(m), k(cat) and k(cat)/K(m)) for the hydrolysis of maltooligosaccharides, starch and glycogen are reported for ileal MG. Maltotriose and maltotetraose displayed partial inhibition of ileal MG. The study revealed large similarities between ostrich SI and MG in charge, size, shape and hydrophobicity, based on their inseparability by several methods. Measurement of the specificity constants for maltooligosaccharide hydrolysis by ileal MG revealed less efficient hydrolysis of longer substrates as compared to maltose and maltotriose.

    Original languageEnglish
    Pages (from-to)339-352
    Number of pages14
    JournalInternational Journal of Biochemistry and Cell Biology
    Volume30
    Issue number3
    DOIs
    Publication statusPublished - 1998 Mar 1

    Keywords

    • Intestinal glycohydrolases
    • Kinetics
    • Ostrich
    • Purification

    ASJC Scopus subject areas

    • Biochemistry
    • Cell Biology

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