Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification

Riko Nishimura, Makoto Wakabayashi, Kenji Hata, Takuma Matsubara, Shiho Honma, Satoshi Wakisaka, Hiroshi Kiyonari, Go Shioi, Akira Yamaguchi, Noriyuki Tsumaki, Haruhiko Akiyama, Toshiyuki Yoneda

Research output: Contribution to journalArticlepeer-review

117 Citations (Scopus)

Abstract

Endochondral ossification is temporally and spatially regulated by several critical transcription factors, including Sox9, Runx2, and Runx3. Although the molecular mechanisms that control the late stages of endochondral ossification (e.g. calcification) are physiologically and pathologically important, these precise regulatory mechanisms remain unclear. Here, we demonstrate that Osterix is an essential transcription factor for endochondral ossification that functions downstream of Runx2. The global and conditional Osterix-deficient mice studied here exhibited a defect of cartilage-matrix ossification and matrix vesicle formation. Importantly, Osterix deficiencies caused the arrest of endochondral ossification at the hypertrophic stage. Microarray analysis revealed that matrix metallopeptidase 13 (MMP13) is an important target of Osterix. We also showed that there exists a physical interaction between Osterix and Runx2 and that these proteins function cooperatively to induceMMP13during chondrocyte differentiation. Most interestingly, the introduction ofMMP13stimulated the calcification of matrices in Osterix-deficient mouse limb bud cells. Our results demonstrated that Osterix was essential to endochondral ossification and revealed that the physical and functional interaction between Osterix and Runx2 were necessary for the induction of MMP13 during endochondral ossification.

Original languageEnglish
Pages (from-to)33179-33190
Number of pages12
JournalJournal of Biological Chemistry
Volume287
Issue number40
DOIs
Publication statusPublished - 2012 Sep 28
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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