Orientation of DNA thin films fabricated on substrates

Hiromi Kimura-Suda, Fumio Nakamura, Masahiko Hara, Tatsuo Wada, Masatsugu Shimomura, Hiroyuki Sasabe

    Research output: Contribution to journalArticle

    Abstract

    Two types of multifunctional thin films based on DNA polyion complex intercalated with photofunctional chromophore were fabricated on the 2D-surface. One is monolayer of DNA polyion complex transferred to a glass substrate at the air/water interface, and is intercalated with acridine orange (AO). Another is DNA containing thiol moiety, and is intercalated with AO and formed polyion complex with lipid, and is fabricated on a gold substrate at the solid/liquid interface. Molecular orientation of AO was investigated by surface plasmon resonance, polarized UV and FT-IR spectroscopy. AO intercalated DNA polyion complex film (a) was aligned perpendicular to the DNA long axis. In the case of DNA polyion complex film (b), orientated DNA could be fabricated on the gold substrate through S-Au interaction, and the orientation of DNA was more stable by mixing with lipid to provide polyion complex. AO in DNA polyion complex film (b) was aligned parallel to the substrate.

    Original languageEnglish
    Pages (from-to)367-370
    Number of pages4
    JournalMolecular Crystals and Liquid Crystals Science and Technology Section A: Molecular Crystals and Liquid Crystals
    Volume370
    DOIs
    Publication statusPublished - 2001 Jan 1

    Keywords

    • DNA
    • Intercalation
    • Orientation
    • Polyion complex
    • Thin film

    ASJC Scopus subject areas

    • Condensed Matter Physics

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