Organization and localization of the dnaA and dnaK gene regions on the multichromosomal genome of Burkholderia multivorans ATCC 17616

Yuji Nagata, Muneaki Matsuda, Harunobu Komatsu, Yoshiyuki Imura, Hiroyuki Sawada, Yoshiyuki Ohtsubo, Masataka Tsuda

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The Burkholderia multivorans strain ATCC 17616 carries three circular chromosomes with sizes of 3.4, 2.5, and 0.9 Mb. To reveal the distribution and organization of the genes for fundamental cell functions on the genome of this bacterium, the dnaA and dnaK gene regions of ATCC 17616 were cloned and characterized. The gene organization of the dnaA region was rnpA-rmpH-dnaA-dnaN- gyrB with a single consensus DnaA-binding box (TTATCCACA) between the rmpH and dnaA genes. This intergenic region, however, did not work as an autonomously replicating sequence in ATCC 17616. On the other hand, the gene organization of the dnaK region was grpE-orf1 (gene for thioredoxin homologue)-dnaK-dnaJ-pabB (gene for p-aminobenzoate synthetase component homologue). A putative heat-shock promoter that showed good homology to the σ-dependent promoter consensus sequence in Escherichia coli was found upstream of the grpE gene, suggesting that these five genes constitute an operon. In M9 succinate minimal medium the dnaJ mutant grew more slowly than the wild-type strain, indicating that this operon is functional. Pulsed-field gel electrophoresis and Southern blot analyses indicated that both the dnaA and dnaK gene regions exist as single copies on the 3.4 Mb chromosome.

Original languageEnglish
Pages (from-to)603-610
Number of pages8
JournalJournal of Bioscience and Bioengineering
Volume99
Issue number6
DOIs
Publication statusPublished - 2005

Keywords

  • Burkholderia
  • DnaA box
  • Heat shock proteins
  • Replication origin
  • dnaA
  • dnaJ
  • dnaK
  • dnaN
  • gyrB

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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