Organelle Distribution in a Hydrated Bio-cell by Correlation between Soft X-ray and Fluorescence Images

T. Ejima, M. Kado, M. Aoyama, K. Yasuda, S. Tamotsu

    Research output: Contribution to journalConference articlepeer-review

    1 Citation (Scopus)

    Abstract

    Fine structures of bio-specimens obtained by soft X-ray (SX) imaging are clearer compared with those obtained by visible imaging owing to the difference in extinction coefficients between the two wavelength regions. However, it is difficult to identify the fine structure imaged in the 2.3 - 4.5 nm wavelength region by appearance alone. Here, we obtain and compare SX and fluorescence images of Leydig cells of a mouse testis loaded with fluorophores. Identification of the fine structures in the SX image is carried out by comparison with the fluorescence images with the use of principal component analysis (PCA). The result shows that the common structures between the SX- and the fluorescence- images.

    Original languageEnglish
    Article number012009
    JournalJournal of Physics: Conference Series
    Volume849
    Issue number1
    DOIs
    Publication statusPublished - 2017 Jun 14
    Event13th International X-ray Microscopy Conference, XRM 2016 - Oxford, United Kingdom
    Duration: 2016 Aug 152016 Aug 19

    ASJC Scopus subject areas

    • Physics and Astronomy(all)

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