TY - JOUR
T1 - Orexigenic action of oral zinc
T2 - Metabolomic analysis in the rat hypothalamus
AU - Nishiuchi, Mika
AU - Sakai, Kumiko
AU - Tajima, Hiroyuki
AU - Katayama, Kazuo
AU - Kimura, Fumiko
AU - Hoshi, Seiko
AU - Goto, Tomoko
AU - Shirakawa, Hitoshi
AU - Komai, Michio
N1 - Funding Information:
This work was partially supported by the grant of the intramural collaboration research in Shokei Gakuin University (to MN), and the JSPS Core-to-Core Program A (Advanced Research Networks) entitled: “Establishment of international agricultural immunology research-core for a quantum improvement in food safety” (to HS).
Publisher Copyright:
© 2018 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2018
Y1 - 2018
N2 - We previously reported an orexigenic action of oral zinc administration in male Sprague- Dawley (SD) rats during an early stage of feeding with a zinc-deficient diet, without decreased zinc concentrations in tissues. The overall conclusion was that orally but not intraperitoneally administered zinc stimulates food intake in short-term zinc-deficient-diet fed rats. We here investigate the mechanism of the orexigenic action of zinc using GC-MS/MS-targeted metabolomic analysis in the rat hypothalamus. Four-week-old, male SD/Slc rats were used, and after 2 days of feeding with a zinc-deficient diet, 3 mg of ZnSO4 in 5 mL saline solution were administered to each rat either orally or intraperitoneally. Three hours after administration, the rats were sacrificed and the hypothalamus were excised and analyzed. We found that the oral administration group showed increased concentrations of 3-aminopropanoic acid (ß- alanine), hypotaurine, dopamine, and biotin. In light of metabolomic analysis of these results, we indicate directions for further research.
AB - We previously reported an orexigenic action of oral zinc administration in male Sprague- Dawley (SD) rats during an early stage of feeding with a zinc-deficient diet, without decreased zinc concentrations in tissues. The overall conclusion was that orally but not intraperitoneally administered zinc stimulates food intake in short-term zinc-deficient-diet fed rats. We here investigate the mechanism of the orexigenic action of zinc using GC-MS/MS-targeted metabolomic analysis in the rat hypothalamus. Four-week-old, male SD/Slc rats were used, and after 2 days of feeding with a zinc-deficient diet, 3 mg of ZnSO4 in 5 mL saline solution were administered to each rat either orally or intraperitoneally. Three hours after administration, the rats were sacrificed and the hypothalamus were excised and analyzed. We found that the oral administration group showed increased concentrations of 3-aminopropanoic acid (ß- alanine), hypotaurine, dopamine, and biotin. In light of metabolomic analysis of these results, we indicate directions for further research.
KW - GC-MS/MS targeting
KW - Hypothalamus
KW - Metabolomics
KW - Zinc
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U2 - 10.1080/09168451.2018.1516543
DO - 10.1080/09168451.2018.1516543
M3 - Article
C2 - 30240332
AN - SCOPUS:85056321787
VL - 82
SP - 2168
EP - 2175
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 12
ER -