Orexigenic action of oral zinc: Metabolomic analysis in the rat hypothalamus

Mika Nishiuchi, Kumiko Sakai, Hiroyuki Tajima, Kazuo Katayama, Fumiko Kimura, Seiko Hoshi, Tomoko Goto, Hitoshi Shirakawa, Michio Komai

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)


    We previously reported an orexigenic action of oral zinc administration in male Sprague- Dawley (SD) rats during an early stage of feeding with a zinc-deficient diet, without decreased zinc concentrations in tissues. The overall conclusion was that orally but not intraperitoneally administered zinc stimulates food intake in short-term zinc-deficient-diet fed rats. We here investigate the mechanism of the orexigenic action of zinc using GC-MS/MS-targeted metabolomic analysis in the rat hypothalamus. Four-week-old, male SD/Slc rats were used, and after 2 days of feeding with a zinc-deficient diet, 3 mg of ZnSO4 in 5 mL saline solution were administered to each rat either orally or intraperitoneally. Three hours after administration, the rats were sacrificed and the hypothalamus were excised and analyzed. We found that the oral administration group showed increased concentrations of 3-aminopropanoic acid (ß- alanine), hypotaurine, dopamine, and biotin. In light of metabolomic analysis of these results, we indicate directions for further research.

    Original languageEnglish
    Pages (from-to)2168-2175
    Number of pages8
    JournalBioscience, Biotechnology and Biochemistry
    Issue number12
    Publication statusPublished - 2018


    • GC-MS/MS targeting
    • Hypothalamus
    • Metabolomics
    • Zinc

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry


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