Ordering of the flagellar genes in Pseudomonas aeruginosa by insertions of mercury transposon Tn501

M. Tsuda, T. Iino

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The flagellar genes of Pseudomonas aeruginosa PAO cluster on the chromosome at two distinct regions, region I and region II. The order of the flagellar cistrons in this organism was established by using transducing phage G101 and plasmids FP5 and R68.45. A method to insert transposon Tn501 near the fla genes was devised. The authors obtained two strains in which Tn501 was inserted at sites close to the flagellar cistrons in region II. The authors isolated Fla- mutants in which the chromosomal segment between the two Tn501 insertion sites was deleted. Using Tn501-encoded mercury resistance as an outside marker, they determined the order of 9 of the 11 flagellar cistrons in region II as follows: puuF-region I-flaG-flaC-flaI-flaH-flaD-flaB-flaA-flaF-flaE-pur-67. By using phage G101-mediated transduction, the mutation converting monoflagellated bacteria into the multiflagellated (mfl) form was closely linked to the five fla cistrons in region I. Using mfl as an outside marker, they determined the order of the five cistrons as follows: puuF-flaV-flaZ-flaW-flaX-flaY-region II. The mfl mutation was shown to be either located within the flaV cistron or linked very closely to this cistron. No linkage was observed in transductions between any of the fla cistrons in region I and any of the fla cistrons in region II.

Original languageEnglish
Pages (from-to)1008-1017
Number of pages10
JournalJournal of bacteriology
Volume153
Issue number2
Publication statusPublished - 1983 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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