Oral recombinant methioninase prevents obesity in mice on a high-fat diet

Yoshihiko Tashiro, Qinghong Han, Yuying Tan, Norihiko Sugisawa, Jun Yamamoto, Hiroto Nishino, Sachiko Inubushi, Takashi Higuchi, Takeshi Aoki, Masahiko Murakami, Robert M. Hoffman

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Background/Aim: obesity is a world-wide recalcitrant problem leading to many diseases. Dietary methionine restriction (MR) has been shown to prevent obesity, but it is an onerous regimen. The present study aimed to determine the effects of oral recombinant methionase (o-rMETase), on preventing obesity in mice on a high-fat diet. Materials and Methods: Male C57BL/6J mice in the control group were fed a control diet (CD) (+6.5% fat) for 25 days, and others were fed a high-fat (HF) diet (+34.3% fat) for 25 days. Then, the mice were divided into three dietary groups: 1) HF + phosphate buffered saline (PBS) group; 2) HF + o-rMETase group; and 3) untreated non-HF group. Results: The mice on the CD increased in body weight by 14% during experimental period of 25 days; in contrast the mice in the HF+PBS group increased by 33%; however, the mice on the HF+o-rMETase group increased only by 14% (p=0.02, HF+PBS vs HF+o-rMETase). Conclusion: The HF+ o-rMETase group had the same weight increase as untreated mice on a normal fat diet, demonstrating the potential for o-rMETase to eliminate the need for dieting to maintain normal body weight.

Original languageEnglish
Pages (from-to)489-494
Number of pages6
JournalIn Vivo
Issue number2
Publication statusPublished - 2020
Externally publishedYes


  • High fat diet
  • MR
  • Methioninase
  • Methionine restriction
  • Mice
  • Obesity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology


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