Oral MucoRice expressing double-mutant cholera toxin A and B subunits induces toxin-specific neutralising immunity

Yoshikazu Yuki, Daisuke Tokuhara, Tomonori Nochi, Hiroshi Yasuda, Mio Mejima, Shiho Kurokawa, Yuko Takahashi, Nobuhiro Kataoka, Ushio Nakanishi, Yukari Hagiwara, Kohtaro Fujihashi, Fumio Takaiwa, Hiroshi Kiyono

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Rice-expressed cholera toxin B (CTB) subunit is a cold-chain-free oral vaccine that effectively induces enterotoxin-neutralising immunity. We created another rice-based vaccine, MucoRice, expressing nontoxic double-mutant cholera toxin (dmCT) with CTA and CTB subunits. Western-blot analysis suggested that MucoRice-dmCT had the shape of a multicomponent vaccine. Oral administration of MucoRice-dmCT induced CTB- but not CTA-specific serum IgG and mucosal IgA antibodies, generating protective immunity against cholera toxin without inducing rice-protein-specific antibody responses. The potency of MucoRice-dmCT was equal to that of MucoRice-CTB vaccine. MucoRice has the potential to be used as a safe multicomponent vaccine expression system.

Original languageEnglish
Pages (from-to)5982-5988
Number of pages7
JournalVaccine
Volume27
Issue number43
DOIs
Publication statusPublished - 2009 Oct 9
Externally publishedYes

Keywords

  • Cholera
  • Cold-chain-free
  • MucoRice
  • Mutant cholera toxin
  • Oral vaccine
  • Rice-based vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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