@article{ace3cc266d9542c6b683e615d95f0bc7,
title = "Optogenetic activation of the fruitless-labeled circuitry in Drosophila subobscura males induces mating motor acts",
abstract = "It remains an enigma how the nervous system of different animal species produces different behaviors. We studied the neural circuitry for mating behavior in Drosophila subobscura, a species that displays unique courtship actions not shared by other members of the genera including the genetic model D. melanogaster, in which the core courtship circuitry has been identified. We disrupted the D. subobscura fruitless (fru) gene, amasterregulatorforthecourtshipcircuitryformationinD. melanogaster, resultingincompletelossof matingbehavior. We also generated frusoChrimV, which expresses the optogenetic activator Chrimson fused with a fluorescent marker under the native fru promoter. Thefru-labeledcircuitryinD. subobscuravisualizedbyfrusoChrimV revealeddifferences betweenfemales andmales, optogeneticactivation of which in males induced mating behavior including attempted copulation. These findings provide a substrate for neurogenetic dissection and manipulation of behavior in non-model animals, and will help to elucidate the neural basis for behavioral diversification.",
keywords = "CRISPR/Cas9, Courtship, Drosophila, Fruitless",
author = "Ryoya Tanaka and Tomohiro Higuchi and Soh Kohatsu and Kosei Sato and Daisuke Yamamoto",
note = "Funding Information: Received July 9, 2017; revised Oct. 16, 2017; accepted Oct. 18, 2017. Authorcontributions:R.T.andD.Y.designedresearch;R.T.andT.H.performedresearch;S.K.contributedunpub-lished reagents/analytic tools; R.T., T.H., S.K., K.S., and D.Y. analyzed data; R.T. and D.Y. wrote the paper. This work was supported, in part, by Grant-in-Aids for Scientific Research (Nos. 17K19371, 17H05935, and 16H06371)fromTheMinistryofEducation,Culture,Sports,ScienceandTechnology(MEXT)toD.Y.,andaGrant-in-Aid for JSPS fellows (15J05088) to R.T. We thank A. Utsumi for secretarial assistance. The authors declare no competing financial interests. CorrespondenceshouldbeaddressedtoDr.DaisukeYamamoto,DivisionofNeurogenetics,TohokuUniversity,Graduate SchoolofLifeSciences,2-1-1Katahira,Aoba-ku,Sendai980-8577,Japan.E-mail:daichan@m.tohoku.ac.jp. DOI:10.1523/JNEUROSCI.1943-17.2017 Copyright{\textcopyright}2017theauthors 0270-6474/17/3711662-13$15.00/0 Funding Information: This work was supported, in part, by Grant-in-Aids for Scientific Research (Nos. 17K19371, 17H05935, and 16H06371) from The Ministry of Education, Culture, Sports, Science and Technology (MEXT) to D.Y., and a Grant-in-Aid for JSPS fellows (15J05088) to R.T. We thank A. Utsumi for secretarial assistance. Publisher Copyright: {\textcopyright} 2017 the authors.",
year = "2017",
month = nov,
day = "29",
doi = "10.1523/JNEUROSCI.1943-17.2017",
language = "English",
volume = "37",
pages = "11662--11674",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "48",
}