Opto-fMRI analysis for exploring the neuronal connectivity of the hippocampal formation in rats

Yoshifumi Abe, Masaki Sekino, Yasushi Terazono, Hiroyuki Ohsaki, Yugo Fukazawa, Seiichiro Sakai, Hiromu Yawo, Tatsuhiro Hisatsune

    Research output: Contribution to journalArticle

    28 Citations (Scopus)

    Abstract

    In recent years, optical stimulation of neurons that bear a light-gated cation channel, "Optogenetics", has opened a new avenue for exploring neuronal connectivity of the nervous system. In this study, we applied a technique, "Opto-fMRI", which combined optogenetics with blood oxygenation level-dependent (BOLD) functional MRI (fMRI), for examining the neuronal connectivity of the hippocampal formation in rats. Although the hippocampal formation is very important for memory formation and retrieval, there is little information on its neuronal connectivity, especially on its longitudinal axis of connection. For this purpose, we utilized a transgenic rat strain, expressing the light-gated cation channel channelrhodopsin-2 (ChR2) under the regulation of the Thy1.2 promoter which permits the expression of the integrated gene in neurons. After optical stimulation targeting the dentate gyrus of the transgenic rat, we detected BOLD response of not only the dentate gyrus (DG) but also at the CA3 area. In addition, we detected the longitudinal-axis activation of the hippocampus after optical stimulation. Our study suggests that Opto-fMRI could be a tool for exploring the neuronal connectivity of the hippocampal formation, to understand the neural basis of memory formation and retrieval.

    Original languageEnglish
    Pages (from-to)248-255
    Number of pages8
    JournalNeuroscience Research
    Volume74
    Issue number3-4
    DOIs
    Publication statusPublished - 2012 Dec 1

    Keywords

    • Channelrhodopsin-2
    • FMRI
    • Hippocampus
    • Neuronal network
    • Optogenetics
    • Transgenic rat

    ASJC Scopus subject areas

    • Neuroscience(all)

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  • Cite this

    Abe, Y., Sekino, M., Terazono, Y., Ohsaki, H., Fukazawa, Y., Sakai, S., Yawo, H., & Hisatsune, T. (2012). Opto-fMRI analysis for exploring the neuronal connectivity of the hippocampal formation in rats. Neuroscience Research, 74(3-4), 248-255. https://doi.org/10.1016/j.neures.2012.08.007