Optimum conditions of ultrasound-mediated destruction of bubble liposome for siRNA transfer in bladder cancer

Suguru Fujisawa, Hiroshige Arakawa, Ryo Suzuki, Kazuo Maruyama, Tetsuya Kodama, Masahiro Yasunaga, Yoshikatsu Koga, Yasuhiro Matsumura

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background: We investigated the effectiveness of ultrasound-mediated destruction of bubble liposome (UBL) for siRNA transfer by observing reduction in the luciferase activity of human bladder tumor RT-112 cells transfected with the luciferase gene (RT-112Luc) following luciferase siRNA transfer into the cells. Results: siRNA was transferred to 26% of RT-112Luc cells by UBL and the luciferase activity of RT-112Luc cells was significantly suppressed by UBL using the luciferase siRNA, compared with that using nonspecific siRNA in vitro (p = 0.036). The luciferase activity of RT-112Luc tumor was suppressed by UBL using luciferase siRNA compared with that using nonspecific siRNA 2 days after the in vivo treatment. Conclusion: This study showed that UBL is suitable for siRNA transfer to mammalian cells.

Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalTherapeutic Delivery
Volume1
Issue number2
DOIs
Publication statusPublished - 2010 Aug 1

ASJC Scopus subject areas

  • Pharmaceutical Science

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