Optimization of simultaneous analysis of tetrodotoxin, 4-epitetrodotoxin, 4,9-anhydrotetrodotoxin, and 5,6,11-trideoxytetrodotoxin by hydrophilic interaction liquid chromatography-tandem mass spectrometry

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35 Citations (Scopus)

Abstract

Recently, liquid chromatography-mass spectrometry (LC-MS) has been recognized as a useful method to analyze tetrodotoxin (TTX), the primary toxin for puffer fish poisoning. TTX usually exists with its chemically interchangeable analogs, such as 4-epiTTX and 4, 9-anhydroTTX. TTX and 4-epiTTX have the same molecular weight and the same fragmentation pattern by tandem mass spectrometry (MS-MS). In this study, we optimized conditions for hydrophilic interaction liquid chromatography (HILC)-MS-MS to quantitate TTX, 4-epiTTX, 4,9-anhydroTTX, and 5,6,11-trideoxyTTX with good separation. The relationships between the applied amounts of each TTX analog to the HILC-MS-MS instrument and the peak areas showed good linearity in the ranges examined. The lower limits of detection (signal-to-noise ratio = 3) were 64 pg on-column for TTX, 128 pg for both 4-epiTTX and 4,9-anhydroTTX, and 180 pg for 5,6,11-trideoxyTTX using an API 2000 mass spectrometer.

Original languageEnglish
Pages (from-to)61-64
Number of pages4
JournalForensic Toxicology
Volume29
Issue number1
DOIs
Publication statusPublished - 2011 Jan

Keywords

  • 4,9-Anhydrotetrodotoxin
  • 4-Epitetrodotoxin
  • 5,6,11- Trideoxytetrodotoxin
  • Hydrophilic interaction LC-MS-MS
  • Puffer fish
  • Tetrodotoxin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Biochemistry, medical

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