Optimization of a method for the simultaneous extraction of polar and non-polar oxylipin metabolites, dna, rna, small rna, and protein from a single small tissue sample

Yu Hasegawa, Yurika Otoki, Shannon McClorry, Laurynne C. Coates, Rachel L. Lombardi, Ameer Y. Taha, Carolyn M. Slupsky

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Considering the high heterogeneity that can be present in a single tissue sample, extracting all biomolecules from a single and undivided tissue is preferable because it allows direct comparison of results. Here, we combined a modified Folch extraction method with DNA, RNA, small RNA, and protein extraction using two commercial kits, which allowed us to extract polar metabolites and non-polar oxylipin metabolites, DNA, RNA, small RNA, and protein simultaneously from a small tissue sample. The method was validated in terms of quantity and quality of analytes for downstream analyses.

Original languageEnglish
Article number61
Pages (from-to)1-14
Number of pages14
JournalMethods and Protocols
Volume3
Issue number3
DOIs
Publication statusPublished - 2020 Sep

Keywords

  • Analytical chemistry
  • DNA
  • Extraction
  • Metabolites
  • Molecular biology
  • Protein
  • Purification
  • RNA
  • Systems biology
  • Tissue

ASJC Scopus subject areas

  • Biotechnology
  • Structural Biology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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