Optimisation of ultrasound-mediated gene transfer (sonoporation) in skeletal muscle cells

Hai Dong Liang, Qi Long Lu, Shao An Xue, Michael Halliwell, Tetsuya Kodama, David O. Cosgrove, Hans J. Stauss, Terence A. Partridge, Martin J.K. Blomley

Research output: Contribution to journalArticlepeer-review

71 Citations (Scopus)

Abstract

Ultrasound (US) is a promising tool for facilitating direct gene transfer to skeletal muscle, but no systematic optimisation study has been performed. We exposed H2K myoblast cells to US with varying intensity of exposure and duration to evaluate its effect on cell viability and transfection efficiency using as endpoints transfection rate, average fluorescence intensity (fluorescence normalised by the number of transfected cells) and overall expression (the product of transfection rate and average fluorescence intensity) as indices. Cell viability decreased with exposure time and intensity, consistent with previous findings. Optimal setting of US was observed at the range of 0.5 to 1 W cm -2 with duration of 20 s, producing maximum efficiency (transfection = 4.5%) in gene transfection with minimum cell toxicity (cell viability = 83%). Higher intensity alone or in combination with low intensity and long duration did not improve cell viability and transfection. The increase of eGFP (enhanced green fluorescence protein) plasmid concentration up to 200 μg per mL was related to an increase in average fluorescence intensity and overall expression. However, transfection rate saturated when DNA concentration reached 50 μg per mL despite initial increase with DNA concentration. The average fluorescence intensity was linearly proportional to the logarithm of DNA concentration, suggesting a diffusion-based model for DNA uptake under sonoporation. We conclude that low-intensity US irradiation provides a safe and effective alternative for gene delivery. (E-mail: Haidong.liang@imperial.ac.uk)

Original languageEnglish
Pages (from-to)1523-1529
Number of pages7
JournalUltrasound in Medicine and Biology
Volume30
Issue number11
DOIs
Publication statusPublished - 2004 Nov 1
Externally publishedYes

Keywords

  • Gene therapy
  • Muscle
  • Naked DNA
  • Passive diffusion
  • Ultrasound

ASJC Scopus subject areas

  • Biophysics
  • Radiological and Ultrasound Technology
  • Acoustics and Ultrasonics

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