Optically controlled contraction of photosensitive skeletal muscle cells

Toshifumi Asano, Toru Ishizua, Hiromu Yawo

    Research output: Contribution to journalArticlepeer-review

    36 Citations (Scopus)

    Abstract

    As the skeletal muscle cell is an efficient force transducer, it has been incorporated in bio-microdevices using electrical field stimulation for generating contractile patterns. To improve both the spatial and temporal resolutions, we made photosensitive skeletal muscle cells from murine C2C12 myoblasts, which express channelrhodopsin-2 (ChR2), one of archaea-type rhodopsins derived from green algae Chlamydomonas reinhardtii. The cloned ChR2-expressing C2C12 myoblasts were made and fused with untransfected C2C12 to form multinucleated myotubes. The maturation of myotubes was facilitated by electrical field stimulation. Blue LED light pulse depolarized the membrane potential of a ChR2-expressing myotube and eventually evoked an action potential. It also induced a twitch-like contraction in a concurrent manner. A contraction pattern was thus made with a given pattern of LED pulses. This technique would have many applications in the bioengineering field, such as wireless drive of muscle-powered actuators/microdevices.

    Original languageEnglish
    Pages (from-to)199-204
    Number of pages6
    JournalBiotechnology and Bioengineering
    Volume109
    Issue number1
    DOIs
    Publication statusPublished - 2012 Jan

    Keywords

    • C2C12
    • Channelrhodopsin
    • Muscle contraction
    • Myoblast
    • Myotube
    • Optical stimulation

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

    Fingerprint

    Dive into the research topics of 'Optically controlled contraction of photosensitive skeletal muscle cells'. Together they form a unique fingerprint.

    Cite this