Optical Resolution Photoacoustic Microscopy with Fast Laser Scanning and Fixed Photoacoustic Detector

Kodai Ishikawa, Ryo Shintate, Ryo Nagaoka, Yoshifumi Saijo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Photoacoustic (PA) imaging is rapidly progressing imaging modality in which very short pulsed laser causes thermal expansion to generate PA signal. In previous PA microscope systems, relatively long time was required for image acquisition because they required mechanical scan of the PA transducer. The objective of the present study is to develop fast laser scanning optical resolution photoacoustic microscopy (OR-PAM) with fixed PA signal detector to realize very high frame rate PA imaging. Q-switched Nd:YAG laser with the wavelength of 532 nm, pulse width of 5.5 ns and pulse repetition rate of up to 50 kHz was equipped in the system. Low frequency PA detector was comprised of a glass prism configuring acoustic focusing and a polymethyl methacrylate (PMMA) prism with 5 MHz PZT (lead zirconate titanate) transducer. High frequency PA detector was comprised of the same glass prism and a glass prism with ZnO (zinc oxide) thin film transducer. Galvano scanner operating in the air was controlled by a microcomputer to scan laser beam. PA signal was detected with the fixed PA detector thus realized the frame rate of 5 fps for C-mode equivalent to 500 fps for B-mode. The lateral resolution of the low frequency system was found to be 11.6 μm and the blood vessel on the surface of cod roe was clearly visualized. The system may be applicable for imaging blood flow and vascular dynamics of micro vessel.

Original languageEnglish
Title of host publication40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages4800-4803
Number of pages4
ISBN (Electronic)9781538636466
DOIs
Publication statusPublished - 2018 Oct 26
Event40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018 - Honolulu, United States
Duration: 2018 Jul 182018 Jul 21

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Volume2018-July
ISSN (Print)1557-170X

Other

Other40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018
CountryUnited States
CityHonolulu
Period18/7/1818/7/21

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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