Optical Manipulation of Subcellular Protein Translocation Using a Photoactivatable Covalent Labeling System

Toshiyuki Kowada, Keisuke Arai, Akimasa Yoshimura, Toshitaka Matsui, Kazuya Kikuchi, Shin Mizukami

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The photoactivatable chemically induced dimerization (photo-CID) technique for tag-fused proteins is one of the most promising methods for regulating subcellular protein translocations and protein–protein interactions. However, light-induced covalent protein dimerization in living cells has yet to be established, despite its various advantages. Herein, we developed a photoactivatable covalent protein-labeling technology by applying a caged ligand to the BL-tag system, a covalent protein labeling system that uses mutant β-lactamase. We further developed CBHD, a caged protein dimerizer, using caged BL-tag and HaloTag ligands, and achieved light-induced protein translocation from the cytoplasm to subcellular regions. In addition, this covalent photo-CID system enabled quick protein translocation to a laser-illuminated microregion. These results indicate that the covalent photo-CID system will expand the scope of CID applications in the optical manipulation of cellular functions.

Original languageEnglish
Pages (from-to)11378-11383
Number of pages6
JournalAngewandte Chemie - International Edition
Volume60
Issue number20
DOIs
Publication statusPublished - 2021 May 10

Keywords

  • caged compound
  • dimerization
  • photochemistry
  • proteins
  • protein–protein interactions

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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