One-pot glycosylation reaction from non-protected sugars by chitinase

Atsushi Kobayashi, Michinari Kohri, Takuya Misawa, Hidetoshi Gyakushi, Masato Noguchi, Shin Ichiro Shoda

Research output: Contribution to conferencePaperpeer-review


In order to realize one-pot synthesis of oligosaccharides from non-protected sugar, we employed chemo-enzymatic method using dehydration condensing agent for the activation of non-protected sugar and a bacterial chitinaseA1 from Bacillus circulans WL-12, which belongs to glycosyl hydrolase family 18 recognizing sugar oxazoline. Chitinase-catalyzed glycosylation reactions of sugar oxazoline intermediates prepared in situ by using dehydration condensing agent in aqueous solution using dehydration condensing agent were able to produce corresponding tetrasaccharides when chitobiose 1 and N-acetyllactosamine 2 were employed as starting materials. Therefore, the sequential use of dehydration condensing agent and enzyme is applicable to one-pot oligosaccharide synthesis from non-protected sugars.

Original languageEnglish
Number of pages1
Publication statusPublished - 2006 Dec 1
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: 2006 Sep 202006 Sep 22


Other55th Society of Polymer Science Japan Symposium on Macromolecules


  • Activated sugar
  • Chitinase
  • Dehydration condensing agent
  • Non-protected sugars
  • One-pot glycosylation

ASJC Scopus subject areas

  • Engineering(all)

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