On the use of image quality measures of multi-views in light sheet fluorescence 3D microscopy

Rosa Huaman, Charlotte Riviere, Carole Frindel, Andrea Bassi, David Rousseau

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this report, we discuss the interest of quality metrics for imaging and image processing of multi-views in light sheet fluorescent 3D microscopy. Various metrics of focus are tested on real and simulated data so as to automatically assess the informational quality of the images. Application of such metrics are given for several information tasks including online control of acquisition, fast registration or image fusion. Illustrations are given for typical samples of interest for in vivo imaging with light sheet microscopy such as spheroids or organoids. We point to the reader softwares freely available under Fiji which enable to test the computation of a basic quality metric, for registration and fusion.

Original languageEnglish
Title of host publicationUnconventional Optical Imaging
EditorsCorinne Fournier, Marc P. Georges, Gabriel Popescu
PublisherSPIE
ISBN (Print)9781510618800
DOIs
Publication statusPublished - 2018 Jan 1
Externally publishedYes
EventUnconventional Optical Imaging 2018 - Strasbourg, France
Duration: 2018 Apr 222018 Apr 26

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume10677
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceUnconventional Optical Imaging 2018
CountryFrance
CityStrasbourg
Period18/4/2218/4/26

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Huaman, R., Riviere, C., Frindel, C., Bassi, A., & Rousseau, D. (2018). On the use of image quality measures of multi-views in light sheet fluorescence 3D microscopy. In C. Fournier, M. P. Georges, & G. Popescu (Eds.), Unconventional Optical Imaging [106772V] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10677). SPIE. https://doi.org/10.1117/12.2305844