On-chip electrochemical measurement of β-galactosidase expression using a microbial chip

Takatoshi Kaya, Kuniaki Nagamine, Nobuto Matsui, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

β-Galactosidase expression in a small number of Escherichia coli cells has been measured in real time with an electrochemical sensor chip. E. coli cells were embedded using collagen gel within a micropore which was microfabricated onto a chip. The activity of the expressed β-galactosidase was determined using p-aminophenyl β-d-galactopyranoside (PAPG) as a substrate.

Original languageEnglish
Pages (from-to)248-249
Number of pages2
JournalChemical Communications
Volume4
Issue number2
DOIs
Publication statusPublished - 2004 Jan 1

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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