On an accident by liquid nitrogen - histological changes of skin in cold

Noriko Tabata, Masato Funayama, Takuya Ikeda, Jun ichi Azumi, Masahiko Morita

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Two young researchers were found lying beside 3 Dewar flasks for liquid nitrogen in a cold experimental room of a university. They were sent to a hospital but died 1.5 h after cardiopulmonary resuscitation. One of the cadavers had dark red discoloration of the skin on the left arm at autopsy. By the histological investigation of the discolored region karyopyknosis and vacuolation of the keratinocytes were noticeably observed in the epidermis. In addition, hyperemia and edematous changes were seen. Hemoglobin (Hb) was not immunodetected in the skin tissue except intravascular erythrocytes. Therefore, these histological findings of the discolored skin can indicate that the skin damage was produced by cold due to liquid nitrogen before death. The cause of death was asphyxia due to oxygen deficiency. There were few autopsy findings which showed the participation of liquid nitrogen in the accident. But, the histological findings of the discolored region demonstrate that liquid nitrogen was involved in this accident.

Original languageEnglish
Pages (from-to)61-67
Number of pages7
JournalForensic Science International
Issue number1
Publication statusPublished - 1995 Nov 30
Externally publishedYes


  • Cold
  • Edema
  • Hemoglobin
  • Hyperemia
  • Immunohistochemistry
  • Karyopyknosis and vacuolation in the epidermis
  • Liquid nitrogen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


Dive into the research topics of 'On an accident by liquid nitrogen - histological changes of skin in cold'. Together they form a unique fingerprint.

Cite this