Serial arterial blood sampling is required to measure a blood input function in quantitative estimation of physiological parameters in dynamic positron emission tomography (PET) studies. However, blood sampling is problematic for animal PET study due to limited amount of blood allowed. Therefore, it is of interest to obviate arterial blood sampling. We have previously proposed a method for estimating a blood input curve based on independent component analysis in human study. In this paper, we applied the method to monkey [18F]fluorodeoxyglucose studies, and validated its applicability by comparing the estimated regional cerebral metabolic rates of glucose using the estimated and the measured blood input function. Experimental results suggest that the proposed method enables quantitative analysis in PET without serial arterial blood sampling not only in human data but also in monkey data.