Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA–RNA interface between two structural modules

Ryusei Tsuruga; Narumi Uehara; Yuki Suzuki; Hiroyuki Furuta; Hiroshi Sugiyama; Masayuki Endo; Shigeyoshi Matsumura; Yoshiya Ikawa

doi:10.1016/j.jbiosc.2019.04.003

Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA–RNA interface between two structural modules

Ryusei Tsuruga, Narumi Uehara, Yuki Suzuki, Hiroyuki Furuta, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Bimolecular ribozymes derived by physical dissection of unimolecular ribozymes consisting of two structural modules are promising platforms for the design and construction of assembled RNA nanostructures. Unit RNAs to be assembled intermolecularly into one-dimensional (1D) oligomers are designed by reconnecting the two structural modules in a manner different from the parent ribozymes. This strategy was applied to the Tetrahymena group I ribozyme. We constructed 1D ribozyme oligomers the assembly of which was observed by atomic force microscopy (AFM) and also controlled rationally to design a heterooctamer by differentiating the interface between the two modules.

Original language	English
Pages (from-to)	410-415
Number of pages	6
Journal	Journal of Bioscience and Bioengineering
Volume	128
Issue number	4
DOIs	https://doi.org/10.1016/j.jbiosc.2019.04.003
Publication status	Published - 2019 Oct
Externally published	Yes

Keywords

Catalytic RNA
Group I intron
RNA nanostructure
RNA nanotechnology
Ribozyme

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiosc.2019.04.003

Cite this

Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA–RNA interface between two structural modules. / Tsuruga, Ryusei; Uehara, Narumi; Suzuki, Yuki; Furuta, Hiroyuki; Sugiyama, Hiroshi; Endo, Masayuki; Matsumura, Shigeyoshi; Ikawa, Yoshiya.

In: Journal of Bioscience and Bioengineering, Vol. 128, No. 4, 10.2019, p. 410-415.

Research output: Contribution to journal › Article › peer-review

Tsuruga, Ryusei ; Uehara, Narumi ; Suzuki, Yuki ; Furuta, Hiroyuki ; Sugiyama, Hiroshi ; Endo, Masayuki ; Matsumura, Shigeyoshi ; Ikawa, Yoshiya. / Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA–RNA interface between two structural modules. In: Journal of Bioscience and Bioengineering. 2019 ; Vol. 128, No. 4. pp. 410-415.

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abstract = "Bimolecular ribozymes derived by physical dissection of unimolecular ribozymes consisting of two structural modules are promising platforms for the design and construction of assembled RNA nanostructures. Unit RNAs to be assembled intermolecularly into one-dimensional (1D) oligomers are designed by reconnecting the two structural modules in a manner different from the parent ribozymes. This strategy was applied to the Tetrahymena group I ribozyme. We constructed 1D ribozyme oligomers the assembly of which was observed by atomic force microscopy (AFM) and also controlled rationally to design a heterooctamer by differentiating the interface between the two modules.",

keywords = "Catalytic RNA, Group I intron, RNA nanostructure, RNA nanotechnology, Ribozyme",

author = "Ryusei Tsuruga and Narumi Uehara and Yuki Suzuki and Hiroyuki Furuta and Hiroshi Sugiyama and Masayuki Endo and Shigeyoshi Matsumura and Yoshiya Ikawa",

note = "Funding Information: This work was supported by MEXT KAKENHI Grant Number JP15K05561 (Y.I.). This work was also supported partly by University of Toyama Discretionary Funds of the President “Toyama RNA Research Alliance” (to Y.I. and S.M.). Funding Information: This work was supported by MEXT KAKENHI Grant Number JP15K05561 (Y.I.). This work was also supported partly by University of Toyama Discretionary Funds of the President ?Toyama RNA Research Alliance? (to Y.I. and S.M.). Publisher Copyright: {\textcopyright} 2019 The Society for Biotechnology, Japan",

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AU - Furuta, Hiroyuki

AU - Sugiyama, Hiroshi

AU - Endo, Masayuki

AU - Matsumura, Shigeyoshi

AU - Ikawa, Yoshiya

N1 - Funding Information: This work was supported by MEXT KAKENHI Grant Number JP15K05561 (Y.I.). This work was also supported partly by University of Toyama Discretionary Funds of the President “Toyama RNA Research Alliance” (to Y.I. and S.M.). Funding Information: This work was supported by MEXT KAKENHI Grant Number JP15K05561 (Y.I.). This work was also supported partly by University of Toyama Discretionary Funds of the President ?Toyama RNA Research Alliance? (to Y.I. and S.M.). Publisher Copyright: © 2019 The Society for Biotechnology, Japan

PY - 2019/10

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N2 - Bimolecular ribozymes derived by physical dissection of unimolecular ribozymes consisting of two structural modules are promising platforms for the design and construction of assembled RNA nanostructures. Unit RNAs to be assembled intermolecularly into one-dimensional (1D) oligomers are designed by reconnecting the two structural modules in a manner different from the parent ribozymes. This strategy was applied to the Tetrahymena group I ribozyme. We constructed 1D ribozyme oligomers the assembly of which was observed by atomic force microscopy (AFM) and also controlled rationally to design a heterooctamer by differentiating the interface between the two modules.

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KW - Catalytic RNA

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KW - RNA nanotechnology

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Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA–RNA interface between two structural modules

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Keywords

ASJC Scopus subject areas

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