Abstract
Bimolecular ribozymes derived by physical dissection of unimolecular ribozymes consisting of two structural modules are promising platforms for the design and construction of assembled RNA nanostructures. Unit RNAs to be assembled intermolecularly into one-dimensional (1D) oligomers are designed by reconnecting the two structural modules in a manner different from the parent ribozymes. This strategy was applied to the Tetrahymena group I ribozyme. We constructed 1D ribozyme oligomers the assembly of which was observed by atomic force microscopy (AFM) and also controlled rationally to design a heterooctamer by differentiating the interface between the two modules.
Original language | English |
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Pages (from-to) | 410-415 |
Number of pages | 6 |
Journal | Journal of Bioscience and Bioengineering |
Volume | 128 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2019 Oct |
Externally published | Yes |
Keywords
- Catalytic RNA
- Group I intron
- RNA nanostructure
- RNA nanotechnology
- Ribozyme
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology