Oligo-capped cDNAs for promoter identification and annotation

S. Sugano, Y. Suzuki, R. Yamashita, K. Nakai

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In order to obtain precise information of 5′ end of mRNAs, we have developed the oligo-capping method that replaces the cap of mRNA with synthetic oligonucleotides. Using the data from oligo-capped cDNAs, we constructed database of transcriptional start sites (DBTSS; http://elmo.ims.u-tokyo.ac.jp/dbtss/). This database shows the information on distribution of transcriptional start sites and can be used to identify the core promoter region. Here we show the summery and the update of DBTSS and its future use on identification of regulatory motifs within the promoter.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalInternational Congress Series
Volume1246
Issue numberC
DOIs
Publication statusPublished - 2002 Dec 1

Keywords

  • Database
  • Full-length cDNA
  • Genome
  • Promoter
  • Transcription start

ASJC Scopus subject areas

  • Medicine(all)

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