Oil-free hyaluronic acid matrix for serial femtosecond crystallography

Michihiro Sugahara, Changyong Song, Mamoru Suzuki, Tetsuya Masuda, Shigeyuki Inoue, Takanori Nakane, Fumiaki Yumoto, Eriko Nango, Rie Tanaka, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, So Iwata

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Å resolution.

Original languageEnglish
Article number24484
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Apr 18

ASJC Scopus subject areas

  • General

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