Occurrence of Two Types of Ca2+‐Dependent Protein Kinases in the Cytosol Fraction of the Brain

E. Miyamoto, K. Fukunaga, K. Matsui, Y. Iwasa

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Abstract— Two types of Ca2+‐dependent protein kinases were demonstrated and partially purified from the cytosol fraction of rat brain by DEAE‐cellulose, Sephadex G‐200, and calmodulin‐affinity column chromatography, using endogenous proteins and chicken gizzard myosin light chains as substrates. The molecular weights of the enzymes were 88,000 (peak I) and 120,000 (peak II) on gel filtration. Peak I had no affinity for calmodulin, whereas peak II had a high affinity for it, with a Ka value of 16.7 nm. The Ka values of peaks I and II for Ca2+ were 2.4 and 1.6 μm, respectively.

Original languageEnglish
Pages (from-to)1324-1330
Number of pages7
JournalJournal of Neurochemistry
Volume37
Issue number5
DOIs
Publication statusPublished - 1981 Nov
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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