Occurrence of Multiple Lectins in the Hemolymph of Kuruma Prawn Penaeus japonicus

Koji Muramoto, Takuya Matsuda, Keisuke Nakada

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    The hemolymph of kuruma prawn Penaeusjaponicus agglutinated horse, sheep, chicken, and human erythrocytes, but not a marine bacterium Vibrio anguillamm. The hemagglutinating activity was dependent on the presence of calcium ions. The higher titer values were obtained by keeping the reaction mixture at 6°C for 16 h compared to those observed at room temperature. The hemagglutinating activity was inhibited by glycoproteins and simple sugars such as mucin Type I, mucin Type II, fetuin, D-mannosamine, N-acetyl-D-glucosamine, and iV-acetylneuraminic acid. The lectins were purified by affinity chromatography on mucin Type II-Sepharose 6B. Succeeding separation by ion-exchange chromatography and gel-filtration revealed that the affinity purified lectins were a mixture of the lectins with different molecular species and also sugar-binding specificity. The two lectins were obtained in electrophoreti-cally pure form; one (Fraction B-l) was a protein of 370kDa consisted of subunits of 84kDa and the other (Fraction D, 240 kDa) consisted with subunits of 34 kDa.

    Original languageEnglish
    Pages (from-to)131-135
    Number of pages5
    JournalFisheries Science
    Volume61
    Issue number1
    DOIs
    Publication statusPublished - 1995

    Keywords

    • Penaeus japonicus
    • agglutinins
    • hemagglutinins
    • hemolymph
    • kuruma prawn
    • lectins

    ASJC Scopus subject areas

    • Aquatic Science

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