Occurrence of agmatine pathway for putrescine synthesis in Selenomonas ruminatium

Shaofu Liao, Phuntip Poonpairoj, Kyong Cheol Ko, Yumiko Takatuska, Yoshihiro Yamaguchi, Naoki Abe, Jun Kaneko, Yoshiyuki Kamio

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Selenomonas ruminantium synthesizes cadaverine and putrescine from L-lysine and L-ornithine as the essential constituents of its peptidoglycan by a constitutive lysine/ornithine decarboxylase (LDC/ODC). S. ruminantium grew normally in the presence of the specific inhibitor for LDC/ODC, DL-α-difluoromethylornithine, when arginine was supplied in the medium. In this study, we discovered the presence of arginine decarboxylase (ADC), the key enzyme in agmatine pathway for putrescine synthesis, in S. ruminantium. We purified and characterized ADC and cloned its gene (adc) from S. ruminantium chromosomal DNA. ADC showed more than 60% identity with those of LDC/ODC/ADCs from Gram-positive bacteria, but no similarity to that from Gram-negative bacteria. In this study, we also cloned the aguA and aguB genes, encoding agmatine deiminase (AguA) and N-carbamoyl-putrescine amidohydrolase (AguB), both of which are involved in conversion from agmatine into putrescine. AguA and AguB were expressed in S. ruminantium. Hence, we concluded that S. ruminantium has both ornithine and agmatine pathways for the synthesis of putrescine.

Original languageEnglish
Pages (from-to)445-455
Number of pages11
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number2
DOIs
Publication statusPublished - 2008 Mar 13

Keywords

  • Agmatine deiminase
  • Agmatine pathway for putrescine synthesis
  • Arginine decarboxylase
  • N-carbamoyl-putrescine amidohydrolase
  • Selenomonas ruminantium

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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  • Cite this

    Liao, S., Poonpairoj, P., Ko, K. C., Takatuska, Y., Yamaguchi, Y., Abe, N., Kaneko, J., & Kamio, Y. (2008). Occurrence of agmatine pathway for putrescine synthesis in Selenomonas ruminatium. Bioscience, Biotechnology and Biochemistry, 72(2), 445-455. https://doi.org/10.1271/bbb.70550