Occurrence of 11-oxotetrodotoxin in the red-spotted newt, Notophthalmus viridescens, and further studies on the levels of tetrodotoxin and its analogues in the newt's efts

Mari Yotsu-Yamashita, Dietrich Mebs

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

An analogue of tetrodotoxin (TTX), 11-oxoTTX, was semi-purified from the red-spotted newt, Notophthalmus viridescens, and identified by ESI-MS and 1H NMR spectra. The levels of TTX, 11-oxoTTX and 6-epiTTX in early stage of development (efts) of this newt were investigated by a post-column fluorescent-HPLC system, and compared with those of adult newts. The level of 11-oxoTTX in both of adults and efts were remarkably high, almost close to those of TTX, while 6-epiTTX was a minor component in both stages. The level of 6-epiTTX in efts (1.8±1.3 μg/g, SD, n=10) was significantly larger than that in adults (0.51±0.26 μg/g, SD, n=12) (p<0.05), while no significant difference was observed in the levels of TTX and 11-oxoTTX between efts (13±7.4 and 9.1±5.6 μg/g) and adult newts (16±6.3 and 13±6.2 μg/g, respectively) (p>0.05).

Original languageEnglish
Pages (from-to)893-897
Number of pages5
JournalToxicon
Volume41
Issue number7
DOIs
Publication statusPublished - 2003 Jun 1

Keywords

  • 11-oxotetrodotoxin
  • 6-epitetrodotoxin
  • Newt
  • Notophthalmus viridescens
  • Tetrodotoxin

ASJC Scopus subject areas

  • Toxicology

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